Le groupe QUALTECH AGROBIO - A.BIO.C

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Microbiological analysis

Cette liste d’analyses est non-exhaustive, nous restons à votre disposition pour répondre à vos demandes.

 

I- ALIMENTARY MICROBIOLOGY

FACTORS

METHOD / REFERENCE TEXT

ANALYTICAL PERIOD(*)

ACCREDITATION

Counting of micro organisms – routine method
Method using the count of the colonies obtained at 30°C
NF EN ISO 4833 72 H YES
Seeding and counting of micro organisms using the « spiral » system NF V 08-100XP V08-034 48H Spiral YES
General directives for the counting of the lactic mesophile bacteria NF ISO 15214 72 H YES
Counting of the lactic bacteria – Meats and meat-based products NF V 04-503 72 H YES
Counting in anaerobiosis of sulphite reductive bacteria (ASR) using the count of the colonies – Routine method NF V 08-061 24 H YES
Counting of yeasts and moistures using the count of the colonies at 25°C – Routine method NF V 08-059 5 dayss YES
Counting of presumed Enterobacteria using the count of the colonies at 30°C or at 37 °C – Routine method NF V 08-054 24 H YES
Presumed Enterobacteria at 37°C / g (REBECCA environment) AES 10/07 – 01/08 24 H YES
Counting of presumed coliforms – routine method – Method by counting the colonies obtained at 30°C NF V 08-050 24 H 24 H (spiral) YES
Counting of thermotolerant coliforms by counting the colonies at 44°C – Routine method NF V 08-060 24 H YES
Counting of positive ?-glucuronidase Escherichia coli by counting the colonies at 44°C – Routine method NF ISO 16649-2 24 H YES
Positive ?-glucuronidase positive – Escherichia coli / g (REBECCA environment) AES 10/06 – 01/08 24 H YES
Escherichia coli 0157 H7 PCR IQ Check Negative result :24 H – Positive result : up to 6 days
General directives for the Research of Salmonella
(research, isolation/identification and confirmation)
NF EN ISO 6579 Negative result :72H – Positive result : up to 7 days YES
Research of Salmonella by Simple Method for Salmonella (SMS) technique AES 10/04-05/04 Negative result : 48 H – Positive result : up to 5 days YES
Research of Salmonella by IBISA method AES 10/11-07/11 Negative result : 48H – Positive result : up to 5 days YES
Salmonella – Research in 25 g by PCR method IQ CHECKBIORAD 24H
Salmonella – Research in 25 g by Rapid Salmonella method BRD 07/11 – 12/05 Negative result : 48 H YES
Routine method for the positive coagulase Staphylococcus counting by counting the colonies s at 37°CPartie 2 : without confirmation of colonies NF EN ISO 6888-2 48 H YES
Research Enrichment/ isolation and confirmation of positive coagulase Staphylococcus (Giollitti method)
Detection of staphylococcic enterotoxins in dairy products DGAL/SDSSA/SDPRAT N 2012-8065 Anses Maisons Alfort method CAT-BAC 06 24 h YES
Horizontal method for the research and counting of monocytogenic Listeria
Part 1 : Research method
NF EN ISO 11290-1 Negative result : 72 H – Positive result : up to 7 days YES
Horizontal method for the research and counting of monocytogenic Listeria
Part 2 : Counting method
NF EN ISO 11290-2 Negative result : 48 H – Positive result : up to 6 days YES
Research of monocytogenic listeria by ALOA One Day method AES 10/03-09/00 Negative result : 48 H – Positive result : 72 H YES
Counting of monocytogenic Listeria by ALOA Count method AES 10/05-09/06 Negative result : 48 H – Positive result : 72 H YES
Monocytogenic Listeria – Research in 25 g by PCR method IQ CHECKBIORAD 27H
Counting of Pseudomona spp – Meats and meat-based products NF V 04-504 Negative result : 48 H – Positive result : 96 H YES
Presumptive Pseudomona spp. / g ISO 13 720 48 H YES
Counting of Clostridium perfringens using the count of the colonies and confirmation (additional period) – Routine method NF EN ISO 7937 24 H YES
Counting of presumptive Bacillus cereus by counting the colonies at 30°C – Routine method NF EN ISO 7932 48 H YES
Presumptive Bacillus cereus  (BACCARA environment) N°AES 10/10 – 07/10 24 H YES
Horizontal method for the research of Shigella spp NF ISO 21567 Negative result : 72 H – Positive result up to 8 days YES
Research of Campylobacter spp / 25g (mCCD and CASA environments) NF ISO 10 272-1 Negative result : 96 H – Positive result : 8 days YES
Campylobacter counting NF ISO/TS 10 272-2 Negative result : 2 jours – Positive result : 6 days YES
Halophile bacteria research Dussault-Lachance PT01HA01 10 days
Cans stability / pH NFV 08-408 / NF V08-409 7 days YES
Microbiological control of surfaces– Researched germs : total flora – coliforms Agar slides PT01LS01 48 H
Aerobic flora on surface (Agar boxes) Contact box PT01FC01 72 H

 

 

 

 

II –  WATER MICROBIOLOGY

FACTORS

METHOD / REFERENCE TEXT

ANALYTICAL PERIOD (*)

ACCREDITATION

Revivifiable micro organisms at 36°C

NF EN ISO 6222

(T90-401)

48 H

YES

Revivifiable Micro organisms  at 22°C

NF EN ISO 6222

(T90-401)

72 H

YES

Escherichia coli and coliform bacteria

NF EN ISO 9308-1

(T90-414)

48 H

YES

Spores of sulfito-reductive anaeorobic micro-organisms

NF EN 26461-2

(T90-417)

24 H

YES

Enterococcus

NF EN ISO 7899-2

(T90-416)

48 H

YES

Pseudomona aeruginosa

NF EN ISO 16266

(T90-419)

Negative result : 48 H

Negative result : jusqu’à 8 j

YES

Pathogen staphylococcus

XP T90-412

48 H

YES

Legionella and Legionella pneumophila(no centrifugation)

NF T 90-431

Negative result : à partir

de10 j

Negative result : jusqu’à

13 j

YES

 

III– WATER SAMPLING / FIELD MEASURES

FACTORS

METHOD / REFERENCE TEXT

ACCREDITATION

Sampling of waters destined to the human consumption in order to proceed to physical, chemical and microbiological analysis :- sampling 😮 at the resource
o in production
o in distribution
(immediate sampling(take of a single sample))

NF EN ISO 19458

(T90-480)

FD T90-520

YES

Sampling of treated leisure waters(pool waters ….) in order to proceed to physical-chemical and microbiological analysis – quick sampling (take of a single sample)

NF EN ISO 19458

(T90-480)

FD T90-520

YES

Sampling of waters on aero cooling towers and on hot sanitary waters for the Legionella research – immediate sampling (take of a single sample)

NF EN ISO 19458

(T90-480)

FD T90-520

Circulaire legionella nr 2002/243 du 22/04/02

Ministerial decree from 13/12/04 (JO from 13/12/04)

Ministerial decree from 01/02/10

Circulaire legionella nr 2010/448 du 21/12/10

YES

Free and total chlorine and other oxidants on freshwaters

NF EN ISO 7393-2

(T90-037-2)

YES

pH on freshwaters

NF T 90-008

YES

Temperature on freshwaters

Operational mode : PT11TH01

YES

 

 

IV– OTHER SERVICES
* ALIMENTARY HYGIENE
– Hygiene Visit  (duration : 2 hours)
– Hygiene Audit (duration : 3-4 hours)
– Hygiene Training – HACCP  (1/2 day)

 

(*) – Analytical period : period between the beginning of the analysis and the result acquisition.

NB : the returning period of the results is longer than the analytical period (= necessary time for the validation, input and transmission of results)