Microbiological analysis

This is a non exhaustive list of analysis, we remain available to answer your demands.

I- ALIMENTARY MICROBIOLOGY
FACTORS	METHOD / REFERENCE TEXT	*ANALYTICAL PERIOD ()**	ACCREDITATION
Micro organisms counting – Routine method Method using the counting of the colonies obtained at 30°C	NF EN ISO 4833	72 H	YES
Seeding and counting of the micro organisms using the “spiral” system	NF V 08-100XP V08-034	48H Spiral	YES
General directives for the counting of the lactic mesophile bacteria	NF ISO 15214	72 H	YES

Counting in anaerobiosis of sulfite reductive bacteria (ASR) using the count of the colonies – Routine method	NF V 08-061	24 H	YES
Counting of yeasts and moistures using the count of the colonies at 25°C – Routine method	NF V 08-059	5 jours	YES
Counting of presumed Enterobacteria using the count of the colonies at 30°C or at 37 °C – Routine method	NF V 08-054	24 H	YES
Presumed Enterobacteria at 37°C / g (REBECCA environment)	AES 10/07 – 01/08	24 H	YES
Counting of presumed coliforms – routine method using the count of the colonies obtained at 30°C	NF V 08-050	24 H24 H (spiral)	YES
Counting of thermotolerant coliforms by counting the colonies at 44°C – Routine method	NF V 08-060	24 H	YES
Counting of Escherichia coli ?-glucuronidase positive by counting the colonies at 44°C – Routine method	NF ISO 16649-2	24 H	YES
Escherichia coli – ?-glucuronidase positive / g (REBECCA environment)	AES 10/06 – 01/08	24 H	YES
Escherichia coli 0157 H7	PCR IQ Check	Negative result:24 H – Positive result: up to 6 days
General directives for the research of Salmonella (research, isolation/identification and confirmation)	NF EN ISO 6579	Negative result:72H – Positive result: up to 7 days	YES
Research of Salmonella using the Simple Method for Salmonella (SMS) method	AES 10/04-05/04	Negative result: 48 H – Positive result: up to 5 days	YES
Research of Salmonella using the IBISA method	AES 10/11-07/11	Negative result:48H – Positive result: up to 5 days	YES
Salmonella – research in 25 g using PCR method	IQ CHECKBIORAD	24H
Salmonella – research in 25 g using the Rapid Salmonella method	BRD 07/11 – 12/05	Negative result: 48 H	YES
Routine method for the Counting of positive coagulase Staphylococcus using the count of the colonies at 37°C Part 2 : without confirmation of colonies	NF EN ISO 6888-2	48 H	YES
Research increase/ isolation and confirmation of positive coagulase Staphylococcus (Giollitti method)
Detection of staphylococcal enterotoxins in dairy products	DGAL/SDSSA/SDPRAT N 2012-8065 Anses Maisons Alfort method CAT-BAC 06	24 H	YES
Detection of staphylococcal enterotoxins in any product	DGAL/SDSSA/SDPRAT N 2012-8065 Anses Maisons Alfort method CAT-BAC 06	24 h	YES
Horizontal method for the research counting of monocytogenic Listeria Part 1 : Research methods	NF EN ISO 11290-1	Negative result: 72 H – Positive result: up to 7 days	YES
Horizontal method for the research counting of monocytogenic Listeria Part 2 : Counting method	NF EN ISO 11290-2	Negative result: 48 H – Positive result: up to 6 days	YES
Research of monocytogenic Listeria using the ALOA One Day method	AES 10/03-09/00	Negative result: 48 H – Positive result: 72 H	YES
Counting of monocytogenic Listeria by ALOA Count method	AES 10/05-09/06	Negative result: 48 H – Positive result: 72 H	YES
Monocytogenic Listeria – research in 25 g by PCR method	IQ CHECKBIORAD	27H

Presumptive Pseudomonas spp. / g	ISO 13 720	48 H	YES
Counting of Clostridium perfringens by counting of the colonies and confirmation(additional delay) – routine method	NF EN ISO 7937	24 H	YES
Counting of presumptive Bacillus cereus by counting the colonies at 30°C – routine method	NF EN ISO 7932	48 H	YES
Presumptive Bacillus cereus (milieu BACCARA)	N°AES 10/10 – 07/10	24 H	YES
Horizontal method for the research of Shigella spp	NF ISO 21567	Negative result: 72 H – Positive result: up to 8 days	YES
Research of Campylobacter spp / 25g (mCCD and CASA environments)	NF ISO 10 272-1	Negative result: 96 H – Positive result: 8 days	YES
Campylobacter counting	NF ISO/TS 10 272-2	Negative result: 2 jours – Positive result: 6 days	YES
Halophile Bacteria	Dussault-LachancePT01HA01	10 days
Can stability / pH	NFV 08-408 / NF V08-409	7 days	YES
Microbiological control of surfaces – Researched germs : total flora – coliforms	Agar slides PT01LS01	48 H
Aerobic flora on surface (agar boxes)	Contact box PT01FC01	72 H

II – WATER MICROBIOLOGY
FACTORS	METHOD / REFERENCE TEXT	*ANALYTICAL PERIOD ()**	ACCREDITATION
Revivifiable micro organisms at 36°C	NF EN ISO 6222 (T90-401)	48 H	YES
Revivifiable micro organisms at 22°C	NF EN ISO 6222 (T90-401)	72 H	YES
Escherichia coli and coliform bacteria	NF EN ISO 9308-1 (T90-414)	48 H	YES
Spores of anaerobic sulfito-reductive micro organisms	NF EN 26461-2 (T90-417)	24 H	YES
Enterococcus	NF EN ISO 7899-2 (T90-416)	48 H	YES
Pseudomona aeruginosa	NF EN ISO 16266 (T90-419)	Negative result: 48 H Résultat positif : up to 8 days	YES
Pathogen staphylococcus	XP T90-412	48 H	YES
Legionella and Legionella pneumophila(no centrifugation)	NF T 90-431	Negative result: from 10 days Positive result: up to 13 days	YES

III– WATER SAMPLING / FIELD MEASURES
PARAMETRES	METHODE / TEXTE DE REFERENCE	ACCREDITATION
Sampling of waters destined to the human consumption in order to perform microbiological and physico-chemical analysis :- prelevement : o at the resource o during the production o during the distribution (quick sampling (take of a single sample)	NF EN ISO 19458 (T90-480) FD T90-520	YES
Sampling of waters on aero cooling towers and on hot sanitary waters for the research of legionella- quick sampling (take of a single sample)	NF EN ISO 19458 (T90-480) FD T90-520	YES
Water sampling on aero cooling towers and on sanitary hot waters for the legionella research – instant sampling (take of a single sample)	NF EN ISO 19458 (T90-480) FD T90-520 Circular legionella nr 2002/243 du 22/04/02 Ministerial decree from 13/12/04 (JO du 13/12/04) Ministerial decree from 01/02/10 Circular legionella nr 2010/448 du 21/12/10	YES
Free and total chlorine and other oxidants on freshwater	NF EN ISO 7393-2 (T90-037-2)	YES
pH on freshwaters	NF T 90-008	YES
Temperature on freshwaters	Operational mode: PT11TH01	YES

IV– OTHER SERVICES

* ALIMENTARY HYGIENE

– Hygiene visit (duration : 2 hours)

– Hygiene audit (duration : 3-4 hours)

– Hygiene training – HACCP (1/2 day)

(*) – analytical period : period between the beginning of the analysis and the obtaining of the result

NB : The period to get the results is higher than the analytical period (= time needed for the validation, input and transmission of the results)