Microbiological analysis
This is a non exhaustive list of analysis, we remain available to answer your demands.
I- ALIMENTARY MICROBIOLOGY |
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FACTORS |
METHOD / REFERENCE TEXT |
ANALYTICAL PERIOD (*) |
ACCREDITATION |
Micro organisms counting – Routine method
Method using the counting of the colonies obtained at 30°C |
NF EN ISO 4833 | 72 H | YES |
Seeding and counting of the micro organisms using the “spiral” system | NF V 08-100XP V08-034 | 48H Spiral | YES |
General directives for the counting of the lactic mesophile bacteria | NF ISO 15214 | 72 H | YES |
Counting in anaerobiosis of sulfite reductive bacteria (ASR) using the count of the colonies – Routine method | NF V 08-061 | 24 H | YES |
Counting of yeasts and moistures using the count of the colonies at 25°C – Routine method | NF V 08-059 | 5 jours | YES |
Counting of presumed Enterobacteria using the count of the colonies at 30°C or at 37 °C – Routine method | NF V 08-054 | 24 H | YES |
Presumed Enterobacteria at 37°C / g (REBECCA environment) | AES 10/07 – 01/08 | 24 H | YES |
Counting of presumed coliforms – routine method using the count of the colonies obtained at 30°C | NF V 08-050 | 24 H24 H (spiral) | YES |
Counting of thermotolerant coliforms by counting the colonies at 44°C – Routine method | NF V 08-060 | 24 H | YES |
Counting of Escherichia coli ?-glucuronidase positive by counting the colonies at 44°C – Routine method | NF ISO 16649-2 | 24 H | YES |
Escherichia coli – ?-glucuronidase positive / g (REBECCA environment) | AES 10/06 – 01/08 | 24 H | YES |
Escherichia coli 0157 H7 | PCR IQ Check | Negative result:24 H – Positive result: up to 6 days | |
General directives for the research of Salmonella (research, isolation/identification and confirmation) | NF EN ISO 6579 | Negative result:72H – Positive result: up to 7 days | YES |
Research of Salmonella using the Simple Method for Salmonella (SMS) method | AES 10/04-05/04 | Negative result: 48 H – Positive result: up to 5 days | YES |
Research of Salmonella using the IBISA method | AES 10/11-07/11 | Negative result:48H – Positive result: up to 5 days | YES |
Salmonella – research in 25 g using PCR method | IQ CHECKBIORAD | 24H | |
Salmonella – research in 25 g using the Rapid Salmonella method | BRD 07/11 – 12/05 | Negative result: 48 H | YES |
Routine method for the Counting of positive coagulase Staphylococcus using the count of the colonies at 37°C
Part 2 : without confirmation of colonies |
NF EN ISO 6888-2 | 48 H | YES |
Research increase/ isolation and confirmation of positive coagulase Staphylococcus (Giollitti method) | |||
Detection of staphylococcal enterotoxins in dairy products | DGAL/SDSSA/SDPRAT N 2012-8065 Anses Maisons Alfort method CAT-BAC 06 | 24 H | YES |
Detection of staphylococcal enterotoxins in any product | DGAL/SDSSA/SDPRAT N 2012-8065 Anses Maisons Alfort method CAT-BAC 06 | 24 h | YES |
Horizontal method for the research counting of monocytogenic Listeria
Part 1 : Research methods |
NF EN ISO 11290-1 | Negative result: 72 H – Positive result: up to 7 days | YES |
Horizontal method for the research counting of monocytogenic Listeria
Part 2 : Counting method |
NF EN ISO 11290-2 | Negative result: 48 H – Positive result: up to 6 days | YES |
Research of monocytogenic Listeria using the ALOA One Day method | AES 10/03-09/00 | Negative result: 48 H – Positive result: 72 H | YES |
Counting of monocytogenic Listeria by ALOA Count method | AES 10/05-09/06 | Negative result: 48 H – Positive result: 72 H | YES |
Monocytogenic Listeria – research in 25 g by PCR method | IQ CHECKBIORAD | 27H | |
Presumptive Pseudomonas spp. / g | ISO 13 720 | 48 H | YES |
Counting of Clostridium perfringens by counting of the colonies and confirmation(additional delay) – routine method | NF EN ISO 7937 | 24 H | YES |
Counting of presumptive Bacillus cereus by counting the colonies at 30°C – routine method | NF EN ISO 7932 | 48 H | YES |
Presumptive Bacillus cereus (milieu BACCARA) | N°AES 10/10 – 07/10 | 24 H | YES |
Horizontal method for the research of Shigella spp | NF ISO 21567 | Negative result: 72 H – Positive result: up to 8 days | YES |
Research of Campylobacter spp / 25g (mCCD and CASA environments) | NF ISO 10 272-1 | Negative result: 96 H – Positive result: 8 days | YES |
Campylobacter counting | NF ISO/TS 10 272-2 | Negative result: 2 jours – Positive result: 6 days | YES |
Halophile Bacteria | Dussault-LachancePT01HA01 | 10 days | |
Can stability / pH | NFV 08-408 / NF V08-409 | 7 days | YES |
Microbiological control of surfaces – Researched germs : total flora – coliforms | Agar slides PT01LS01 | 48 H | |
Aerobic flora on surface (agar boxes) | Contact box PT01FC01 | 72 H |
II – WATER MICROBIOLOGY |
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FACTORS |
METHOD / REFERENCE TEXT |
ANALYTICAL PERIOD (*) |
ACCREDITATION |
Revivifiable micro organisms at 36°C |
NF EN ISO 6222 (T90-401) |
48 H |
YES |
Revivifiable micro organisms at 22°C |
NF EN ISO 6222 (T90-401) |
72 H |
YES |
Escherichia coli and coliform bacteria |
NF EN ISO 9308-1 (T90-414) |
48 H |
YES |
Spores of anaerobic sulfito-reductive micro organisms |
NF EN 26461-2 (T90-417) |
24 H |
YES |
Enterococcus |
NF EN ISO 7899-2 (T90-416) |
48 H |
YES |
Pseudomona aeruginosa |
NF EN ISO 16266 (T90-419) |
Negative result: 48 H Résultat positif : up to 8 days |
YES |
Pathogen staphylococcus |
XP T90-412 |
48 H |
YES |
Legionella and Legionella pneumophila(no centrifugation) |
NF T 90-431 |
Negative result: from 10 days Positive result: up to 13 days |
YES |
III– WATER SAMPLING / FIELD MEASURES |
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PARAMETRES |
METHODE / TEXTE DE REFERENCE |
ACCREDITATION
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Sampling of waters destined to the human consumption in order to perform microbiological and physico-chemical analysis :- prelevement :
o at the resource (quick sampling (take of a single sample) |
NF EN ISO 19458 (T90-480) FD T90-520
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YES
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Sampling of waters on aero cooling towers and on hot sanitary waters for the research of legionella- quick sampling (take of a single sample) |
NF EN ISO 19458 (T90-480) FD T90-520
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YES
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Water sampling on aero cooling towers and on sanitary hot waters for the legionella research – instant sampling (take of a single sample) |
NF EN ISO 19458 (T90-480) FD T90-520 Circular legionella nr 2002/243 du 22/04/02 Ministerial decree from 13/12/04 (JO du 13/12/04) Ministerial decree from 01/02/10 Circular legionella nr 2010/448 du 21/12/10
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YES
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Free and total chlorine and other oxidants on freshwater |
NF EN ISO 7393-2 (T90-037-2) |
YES |
pH on freshwaters |
NF T 90-008 |
YES |
Temperature on freshwaters |
Operational mode: PT11TH01 |
YES |
IV– OTHER SERVICES |
* ALIMENTARY HYGIENE |
– Hygiene visit (duration : 2 hours) |
– Hygiene audit (duration : 3-4 hours) |
– Hygiene training – HACCP (1/2 day) |
(*) – analytical period : period between the beginning of the analysis and the obtaining of the result
NB : The period to get the results is higher than the analytical period (= time needed for the validation, input and transmission of the results)